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Novel strategy for anti‐HIV‐1 action: selective cytotoxic effect of N ‐myristoyltransferase inhibitor on HIV‐1‐infected cells
Author(s) -
Takamune Nobutoki,
Hamada Hirotoshi,
Misumi Shogo,
Shoji Shozo
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03199-x
Subject(s) - cytotoxic t cell , cell culture , human immunodeficiency virus (hiv) , virology , biology , gene isoform , mode of action , cell , biochemistry , gene , in vitro , genetics
N ‐myristoyltransferase (NMT) is essential for the survival of eukaryotes and the production of infectious human immunodeficiency virus type‐1(HIV‐1) by the host cell. In this study, we found decreases in the mRNA levels of human NMT isoforms and the NMT activities in the course of HIV‐1 infection in the human T‐cell line, CEM. Investigating the cytotoxic effect of the novel synthetic NMT inhibitors on the chronic HIV‐1 infected T‐cell line, CEM/LAV‐1, and the uninfected CEM, revealed that the cytotoxic effect was significantly selective for CEM/LAV‐1. This was thought to be due to the difference between the NMT levels of the cell lines. In this paper, we propose that NMT may be a candidate target for anti‐HIV‐1‐infected‐cell agents.