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Involvement of Elk‐1 in L6E9 skeletal muscle differentiation
Author(s) -
Khurana Ashwani,
Dey Chinmoy S
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03192-7
Subject(s) - skeletal muscle , mapk/erk pathway , p38 mitogen activated protein kinases , microbiology and biotechnology , cellular differentiation , protein kinase a , phosphorylation , chemistry , kinase , biology , endocrinology , biochemistry , gene
In L6E9 skeletal muscle cells ternary complex factor (TCF) Elk‐1 expression increased with the onset of skeletal muscle differentiation, whereas its activation decreased as a function of differentiation. Its expression was predominantly restricted to cytoplasm and activated ones were predominantly restricted to the nucleus of the differentiated cells. Inhibition of ERK‐1/‐2 activities by PD098059 resulted into significant reduction in Elk‐1 expression and phosphorylation during differentiation. In contrast, inhibition of p38 mitogen‐activated protein kinase (MAPK) enhanced Elk‐1 expression and activation, thereby mediating inhibition of skeletal muscle differentiation. Overexpression of inactive mutant Elk‐1 enhanced differentiation. Data suggest that ERK‐1/‐2 and p38 MAPK activities modulate Elk‐1 expression and activation to regulate skeletal muscle differentiation.