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Proteasomal activity modulates TGF‐ß signaling in a gene‐specific manner
Author(s) -
Zhang Fan,
Mönkkönen Mia,
Roth Stina,
Laiho Marikki
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03163-0
Subject(s) - mg132 , proteasome , transforming growth factor , microbiology and biotechnology , signal transduction , smad , biology , chemistry , proteasome inhibitor
Transforming growth factor‐β (TGF‐β) signaling relies on Smad‐signaling pathway controlled in part by the proteasome. Here we demonstrate that inhibition of the proteasome function in mink epithelial cells accumulates both positive and negative modulators of TGF‐β signaling, phospho‐Smad2 and SnoN. Inhibition of the proteasome led to abrogation of TGF‐β target gene regulation in a gene‐specific manner. While regulation of p15Ink4b and myc by TGF‐β are lost, PAI‐1 induction, previously shown to occur in a Smad3‐dependent manner, was not affected by treatment of the cells with the proteasomal inhibitor MG132. The results suggest that proteasomal activity is required for TGF‐β signaling in a gene‐specific manner.