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Restricted feeding induces daily expression of clock genes and Pai‐1 mRNA in the heart of Clock mutant mice
Author(s) -
Minami Yoichi,
Horikawa Kazumasa,
Akiyama Masashi,
Shibata Shigenobu
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03159-9
Subject(s) - clock , biology , gene expression , messenger rna , mutant , gene , circadian rhythm , endocrinology , medicine , genetics
Plasminogen activator inhibitor‐1 (PAI‐1) is a key factor of fibrinolytic activity. The activity and mRNA abundance show a daily rhythm. To elucidate the mechanism of daily Pai‐1 gene expression, the expression of Pai‐1 and several clock genes was examined in the heart of homozygous Clock mutant ( Clock / Clock ) mice. Damping of the daily oscillation of Pai‐1 gene expression in Clock / Clock mice was accompanied with damped or attenuated oscillations of mPer1 , mPer2 , mBmal1 , and mNpas2 mRNA. Daily restricted feeding induced a daily mRNA rhythm of all clock genes and Pai‐1 mRNA in Clock / Clock mice as well as wild‐type mice. The peaks of clock genes and Pai‐1 mRNA were phase‐advanced in the heart of both genotypes after 6 days of restricted feeding. The present results demonstrate that daily Pai‐1 gene expression depends on clock gene expression in the heart and that a functional Clock gene is not required for restricted feeding‐induced resetting of the peripheral clock.