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Characterization and thermodynamic properties of quadruplex/duplex competition
Author(s) -
Li Wei,
Wu Peng,
Ohmichi Tastuo,
Sugimoto Naoki
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03118-6
Subject(s) - duplex (building) , g quadruplex , chemistry , chemical stability , in vivo , dna , metal , biophysics , stereochemistry , biochemistry , biology , genetics , organic chemistry
Structural characteristics and thermodynamic properties of dG 3 (T 2 AG 3 ) 3 , d(C 3 TA 2 ) 3 C 3 and dG 3 (T 2 AG 3 ) 3 /d(C 3 TA 2 ) 3 C 3 were intensively investigated. It was indicated that metal ions greatly affected the conformation and stability of the G‐quadruplex. A competition of a structure transition among the G‐quadruplex, I‐motif, and the duplex was confirmed to be dependent on both cation species and pH values. The structural competitive mechanism is discussed for the first time. This study shows an intriguing potential in modulating DNA structures in vivo, which is of great importance in drug design and cancer chemotherapy.