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Retardation of the G2‐M phase progression on gene disruption of RNA binding protein Sam68 in the DT40 cell line 1
Author(s) -
Li Qing-Hua,
Haga Izumi,
Shimizu Toshiki,
Itoh Michiyasu,
Kurosaki Tomohiro,
Fujisawa Jun-ichi
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03103-4
Subject(s) - cyclin dependent kinase 1 , mitosis , rna binding protein , biology , cell cycle , microbiology and biotechnology , rna , kinase , cyclin dependent kinase 2 , gene , biochemistry
Sam68 is an RNA binding protein that is tyrosine‐phosphorylated by Src during mitosis and has been postulated to have a role in cell cycle control by modulating RNA metabolism. To elucidate the function of this protein, we isolated a Sam68‐deficient DT40 cell line by gene disruption. The Sam68‐deficient cells exhibited markedly decreased growth and the growth retardation was due to elongation of the G2‐M phase, however, the kinase activity associated with Cdc2 remained unaltered. Our results indicate that Sam68 may play a critical role in G2‐M progression in a manner independent of the control of Cyclin/Cdc2 kinase activity.