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A role for the octameric ring protein, Translin, in mitotic cell division
Author(s) -
Ishida Reiko,
Okado Haruo,
Sato Hiroko,
Shionoiri Chieko,
Aoki Katsunori,
Kasai Masataka
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03095-8
Subject(s) - cytokinesis , mitosis , microbiology and biotechnology , biology , cell cycle , metaphase , cell division , centrosome , multipolar spindles , prophase , spindle apparatus , cell , genetics , chromosome , meiosis , gene
The octameric ring protein, Translin, demonstrates marked similarities to the family of helicase enzymes regarding its quaternary organization and dimerization of subunits. Here we show that the level of Translin closely parallels the proliferative state in various cell types. Expression is periodic during the cell cycle, with protein synthesis becoming maximal in the S and mitosis phases, consistent with a role in cell division. Moreover, induced overexpression of Translin was found to accelerate cell proliferation. Confocal microscopic analysis revealed that Translin is localized at the centrosomes at prophase and the mitotic spindle at metaphase, then translocating to the spindle midbodies during cytokinesis. This novel localization is attributable to specific interactions with microtubules of the mitotic spindles, and especially γ‐tubulin. The results suggest that Translin participates in processes ensuring the segregation of chromosomes and cytokinesis.