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Biochemical properties of platelet microparticle membranes formed during exocytosis resemble organelles more than plasma membrane
Author(s) -
Olas Beata,
Lundell Kerstin,
Holmsen Holm,
Fukami Miriam H
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03060-0
Subject(s) - exocytosis , membrane , organelle , microparticle , platelet , chemistry , biophysics , platelet rich plasma , microbiology and biotechnology , biochemistry , chemical engineering , biology , immunology , engineering
Studies of [ 3 H]glycerol turnover in phosphatidylcholine (PC) in platelets revealed two metabolic pools, a ‘low turnover PC’ in collagen‐induced microparticles with specific radioactivity only 10% of that found in the ‘high turnover PC’ of bulk platelet PC. Isolated organelle fractions of [ 3 H]glycerol‐labelled platelets contained [ 3 H]PC with specific radioactivities about 20% of that in membrane fractions. These results together with studies on distribution of concanavalin A‐FITC and GPlb, a plasma membrane receptor, indicate that microparticles formed during exocytosis are not simple vesiculations of plasma membrane, but they seem rather to originate from a relatively metabolically static membrane pool not accessible to extracellular reagents.