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Enterohemolysin operon of Shiga toxin‐producing Escherichia coli : a virulence function of inflammatory cytokine production from human monocytes
Author(s) -
Taneike Ikue,
Zhang Hui-Min,
Wakisaka-Saito Noriko,
Yamamoto Tatsuo
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03027-2
Subject(s) - escherichia coli , microbiology and biotechnology , operon , virulence , hemolysin , biology , virulence factor , shiga like toxin , toxin , shiga toxin , gene , enterobacteriaceae , genetics
Shiga toxin‐producing Escherichia coli (STEC) is associated with hemolytic uremic syndrome (HUS). Although most clinical isolates of STEC produce hemolysin (called enterohemolysin), the precise role of enterohemolysin in the pathogenesis of STEC infections is unknown. Here we demonstrated that E. coli carrying the cloned enterohemolysin operon ( hlyC , A , B , D genes) from an STEC human strain induced the production of interleukin‐1β (IL‐1β) through its mRNA expression but not tumor necrosis factor‐α from human monocytes. No IL‐1β release was observed with an enterohemolysin (HlyA)‐negative, isogenic E. coli strain carrying a mutation in the hlyA gene. The data suggest that enterohemolysin, a pore‐forming toxin, induces the production of IL‐1β, which is one of serum risk markers for HUS.