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Polyene antibiotic amphotericin B in monomolecular layers: spectrophotometric and scanning force microscopic analysis
Author(s) -
Gruszecki Wieslaw I.,
Gagos Mariusz,
Kernen Peter
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03009-0
Subject(s) - monolayer , hypsochromic shift , dipalmitoylphosphatidylcholine , chemistry , polyene , molecule , langmuir , microscopy , crystallography , analytical chemistry (journal) , chromatography , phospholipid , adsorption , organic chemistry , optics , membrane , phosphatidylcholine , biochemistry , physics , fluorescence
Monolayers of amphotericin B (AmB) and monolayers composed of AmB and dipalmitoylphosphatidylcholine (DPPC) were formed at the argon–water interface and deposited on a solid support by means of the Langmuir–Blodgett technique. The hypsochromic shift observed in the absorption spectra of monolayers is indicative of aggregated structures of AmB. The exciton splitting theory allowed us to calculate the distance between neighboring molecules in the aggregates as 7.8 Å. Scanning force microscopy of the AmB monolayers revealed the formation of a homogeneous monolayer composed of molecules separated by a distance of 6–8 Å. Microscopy also reveals the formation of cylindrical structures of AmB with a diameter close to 17 Å (internal diameter close to 6 Å) in the monolayers containing additionally 10 mol% DPPC.