Premium
Heregulin promotes expression and subcellular redistribution of ADP‐ribosylation factor 3
Author(s) -
Li Feng,
Mandal Mahitosh,
Mishra Sandip K,
Barnes Christopher J,
Kumar Rakesh
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02994-0
Subject(s) - adp ribosylation factor , biology , microbiology and biotechnology , golgi apparatus , endoplasmic reticulum
To identify genes whose expression is modulated by heregulin‐β1 (HRG), a regulatory polypeptide for mammary epithelial cells, we performed differential display screening of MCF‐7 cell mRNA. One cDNA clone upregulated by HRG was identical to human ADP‐ribosylation factor 3 (ARF3), a guanine nucleotide‐binding protein functioning in vesicular trafficking, phospholipase D activation and intracellular transport. HRG treatment increased expression of ARF3 mRNA and protein. Also, HRG triggered a rapid redistribution of ARF3, first to cell membranes and then to the nuclear compartment, where ARF3 colocalized with acetylated histone H3 in discrete regions. In addition, the ARF3 protein was developmentally regulated in the mammary gland with the highest levels in virgin and post‐weaning glands. Together, these findings suggest for the first time that stimulation of ARF3 expression, subcellular redistribution and interaction with acetylated histone H3 may play a role in the action of HRG in mammary epithelial cells.