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Zinc accelerates dengue virus type 2‐induced apoptosis in Vero cells
Author(s) -
Shafee Norazizah,
AbuBakar Sazaly
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02991-5
Subject(s) - apoptosis , dna fragmentation , vero cell , dengue virus , fragmentation (computing) , extracellular , virology , biology , cell culture , virus , microbiology and biotechnology , programmed cell death , biochemistry , ecology , genetics
Dengue virus type 2 (DENV‐2) infection induced apoptotic cellular DNA fragmentation in Vero cells within 8 days of infection. The addition of high concentrations of extracellular Zn 2+ but not Ca 2+ , Mg 2+ or Mn 2+ to the cell culture medium hastened the detection of apoptosis to within 4 h after infection. No apoptotic cellular DNA fragmentation was detected in the cell culture treated with Zn 2+ alone or infected with heat‐ or ultraviolet light‐inactivated DENV‐2 in the presence of Zn 2+ . These results suggest that (i) apoptosis is induced in African green monkey kidney cells infected with live DENV‐2 and (ii) the addition of high extracellular Zn 2+ accelerates detection of apoptosis in the DENV‐2‐infected cells.