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Characterisation of a novel mouse liver aldo‐keto reductase AKR7A5
Author(s) -
Hinshelwood Alison,
McGarvie Gail,
Ellis Elizabeth
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02982-4
Subject(s) - aldo keto reductase , reductase , aldehyde reductase , skeletal muscle , biochemistry , kidney , enzyme , spleen , biology , microbiology and biotechnology , chemistry , endocrinology , immunology
We have characterised a novel aldo‐keto reductase (AKR7A5) from mouse liver that is 78% identical to rat aflatoxin dialdehyde reductase AKR7A1 and 89% identical to human succinic semialdehyde (SSA) reductase AKR7A2. AKR7A5 can reduce 2‐carboxybenzaldehyde (2‐CBA) and SSA as well as a range of aldehyde and diketone substrates. Western blots show that it is expressed in liver, kidney, testis and brain, and at lower levels in skeletal muscle, spleen heart and lung. The protein is not inducible in the liver by dietary ethoxyquin. Immunodepletion of AKR7A5 from liver extracts shows that it is one of the major liver 2‐CBA reductases but that it is not the main SSA reductase in this tissue.