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Cytoskeleton‐related trafficking of the EAAC1 glutamate transporter after activation of the G q/11 ‐coupled neurotensin receptor NTS1
Author(s) -
Najimi Mustapha,
Maloteaux Jean-Marie,
Hermans Emmanuel
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02981-2
Subject(s) - neurotensin , neurotensin receptor , biology , microbiology and biotechnology , protein kinase c , cytochalasin , receptor , glutamate receptor , intracellular , signal transduction , biochemistry , endocrinology , cytoskeleton , neuropeptide , cell
The possible modulation of the glutamate transporter EAAC1 by a class A G protein‐coupled receptor was studied in transfected C6 glioma cells stably expressing the high‐affinity neurotensin receptor NTS1. Brief exposure (5 min) to neurotensin increased Na + ‐dependent D ‐[ 3 H]aspartate uptake by about 70%. The effect of neurotensin was found to result from an increase in cell surface expression of EAAC1 and accordingly, cytochalasin D and colchicine were shown to block the effect of neurotensin on aspartate uptake, suggesting that the cytoskeleton participates in this regulation. Neither protein kinase C nor phosphatidylinositol 3‐kinase activities, two intracellular signaling pathways known to modulate EAAC1, was required for EAAC1‐mediated aspartate transport regulation by neurotensin. Together, these results provide evidence for an acute regulation of EAAC1 trafficking after activation of a G protein‐coupled receptor.