Premium
A cytochrome c encoded by the nar operon is required for the synthesis of active respiratory nitrate reductase in Thermus thermophilus
Author(s) -
Zafra Olga,
Ramı́rez Sandra,
Castán Pablo,
Moreno Renata,
Cava Felipe,
Vallés Cristina,
Caro Eddy,
Berenguer José
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02953-8
Subject(s) - thermus thermophilus , operon , biochemistry , nitrate reductase , reductase , biology , mutant , cytochrome , respiratory chain , enzyme , chemistry , gene , escherichia coli
A cytochrome c (NarC) is encoded as the first gene of the operon for nitrate respiration in Thermus thermophilus . NarC is required for anaerobic growth and for the synthesis of active nitrate reductase (NR). The α and δ subunits (NarG, NarJ) of the NR were constitutively expressed in narC::kat mutants, but NarG appeared in the soluble fraction instead of associated with the membranes. Our data demonstrate for NarC an essential role in the synthesis of active enzyme and for the attachment to the membrane of the respiratory NR from T. thermophilus .