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Hypoxia activates a background conductance in freshly isolated pulmonary arterial endothelial cells of the rat
Author(s) -
Hogg Dayle S,
Kozlowski Roland Z
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02912-5
Subject(s) - hypoxic pulmonary vasoconstriction , hypoxia (environmental) , electrophysiology , vasoconstriction , chemistry , patch clamp , pharmacology , medicine , biophysics , oxygen , biology , organic chemistry
Utilising the patch‐clamp recording technique we have demonstrated for the first time the effects of hypoxia on the background current in pulmonary arterial endothelial cells. Electrophysiological studies revealed the presence of a novel oxygen‐sensitive, non‐selective cation conductance ( I NSC ) in these cells. The inward component of I NSC was significantly potentiated by hypoxia. Both the inward and outward components of I NSC were inhibited by both La 3+ and Gd 3+ . Hypoxic activation of I NSC may provide an important Ca 2+ influx pathway essential for the release of a pulmonary‐selective vasoconstrictor pivotal to the sustained phase of hypoxic pulmonary vasoconstriction.