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Protein disulfide isomerase of Toxoplasma gondii is targeted by mucosal IgA antibodies in humans
Author(s) -
Meek Bob,
Back Jaap Willem,
Klaren Vincent N.A,
Speijer Dave,
Peek Ron
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02911-3
Subject(s) - toxoplasma gondii , protein disulfide isomerase , antibody , disulfide bond , chemistry , secretory iga , immunoglobulin a , virology , immunoglobulin g , immunology , microbiology and biotechnology , biology , biochemistry
Mass spectrometric analysis identified a 49 kDa antigen from Toxoplasma gondii as protein disulfide isomerase (PDI). This antigen is generally recognized by IgA in tears of healthy humans. We determined the complete open reading frame and expressed PDI recombinantly. Recombinant PDI was recognized by IgA in human tears known to contain antibodies specific for the 49 kDa antigen. High expression level and similarity to other protozoan PDIs suggest that T. gondii PDI might be a suitable target for recently described anti‐protozoan drugs. PDI‐specific antibodies clearly constitute part of the mucosal antibody repertoire possibly involved in defence against parasites.