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Temporal and spatial expression of two isoforms of the Dutt1/Robo1 gene in mouse development
Author(s) -
Clark Katherine,
Hammond Ester,
Rabbitts Pamela
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02904-6
Subject(s) - biology , alternative splicing , gene isoform , immunohistochemistry , embryogenesis , gene , gene expression , messenger rna , embryonic stem cell , microbiology and biotechnology , pathology , genetics , immunology , medicine
The mammalian homologue of the Drosophila axonal guidance receptor roundabout is expressed in a wide range of tissues. Here we show that alternative splicing of the Dutt1 / Robo1 gene results in two mRNA transcripts with different signal peptides, which are differentially expressed throughout mouse embryogenesis. Since mice with a targeted deletion in the Dutt1 / Robo1 gene have abnormal lung pathology, immunohistochemistry was used to identify the cellular expression pattern of Dutt1/Robo1 during lung development. Dutt1/Robo1 expression was widespread and diffuse in the lung at embryonic day 17.5 but became increasingly localised to the bronchial epithelium in newborn and adult mice.