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Transcriptional regulation of nuclear orphan receptor, NOR‐1 , by Ca 2+ /calmodulin‐dependent protein kinase cascade
Author(s) -
Inuzuka Hiroyuki,
Tokumitsu Hiroshi,
Ohkura Naganari,
Kobayashi Ryoji
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02890-9
Subject(s) - microbiology and biotechnology , calmodulin , neuron derived orphan receptor 1 , recombinant dna , kinase , protein kinase a , biology , transcription (linguistics) , messenger rna , creb , promoter , chemistry , transcription factor , gene expression , gene , nuclear receptor , biochemistry , enzyme , linguistics , philosophy
We found that transcription of NOR‐1 (neuron‐derived orphan receptor‐1) is induced by the Ca 2+ /calmodulin‐dependent protein kinase IV (CaM‐KIV) cascade by differential hybridization screening of a cDNA array using probes prepared from SH‐SY5Y cells infected with recombinant adenoviruses expressing Ca 2+ /CaM‐independent mutants of CaM‐KIV and CaM‐K kinase (CaM‐KK). Induction of NOR‐1 mRNA expression by the CaM‐KIV cascade was confirmed by RT‐PCR. Promoter analyses indicate that CaM‐KIV cascade response element is located between −162 bp and −42 bp in the 1.7 kb NOR‐1 promoter containing triple cAMP response elements. Disruption of each element significantly reduced the promoter activity, indicating the direct transcriptional regulation of NOR‐1 by CaM‐KIV cascade.