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Quantification of NF1 transcripts reveals novel highly expressed splice variants
Author(s) -
Vandenbroucke Ina,
Vandesompele Jo,
De Paepe Anne,
Messiaen Ludwine
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02887-9
Subject(s) - splice , neurofibromin 1 , gene , biology , genetics , rna splicing , alternative splicing , sequence (biology) , computational biology , microbiology and biotechnology , messenger rna , rna
Previously, we have shown that the NF1 gene gives rise to multiple novel splice variants. In the present study, nine NF1 variants were quantified by real‐time PCR in various human tissues. Some of these variants were expressed at low to moderate low levels and possible implications of these findings are discussed. Interestingly, two variants (NF1‐ΔE4b and NF1‐ΔE43) were shown to be highly expressed in specific tissues. NF1‐ΔE43 lacks a nuclear targeting sequence and might be functionally different from full‐length NF1 . These novel NF1 splice variants might expand our understanding of the role of neurofibromin.