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Naturally occurring heterologous trans ‐splicing of adenovirus RNA with host cellular transcripts during infection
Author(s) -
Kikumori Toyone,
Cote Gilbert J,
Gagel Robert F
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02878-8
Subject(s) - rna splicing , biology , rna , microbiology and biotechnology , viral replication , heterologous , protein splicing , trans splicing , virology , gene , virus , genetics
The impact of viral infection on normal host RNA processing remains largely unexplored. We postulated that the high abundance of virally derived nuclear RNA in infected cells could impact host cell RNA splicing and viability. To test for aberrant RNA splicing we examined trans ‐splicing following infection with the replication‐competent adenovirus mutant d11520 that lacks E1B 55 kDa protein. Trans ‐splicing was observed between viral RNA and several cellular precursor mRNAs, including β‐actin and glyceraldehyde‐3‐phosphate dehydrogenase. Using a tetracycline‐inducible model system simulating viral trans ‐splicing activity we observed that overexpression of a trans ‐splicing RNA specifically inhibited cell proliferation. These results demonstrate that heterologous trans ‐splicing occurs naturally during adenovirus infection and suggest that trans ‐splicing may contribute to disruption of cell function.