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Direct modulation of volume‐regulated anion channels by Ca 2+ chelating agents
Author(s) -
Lemonnier L,
Vitko Y,
Shuba Y.M,
Vanden Abeele F,
Prevarskaya N,
Skryma R
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02863-6
Subject(s) - chelation , bapta , chemistry , blockade , swell , biophysics , biochemistry , inorganic chemistry , receptor , biology , oceanography , geology
Ca 2+ chelating agents are widely used in biological research for Ca 2+ buffering. Here we report that BAPTA, EDTA and HEDTA produce fast, reversible, voltage‐dependent inhibition of swelling‐activated Cl − current ( I Cl,swell ) in LNCaP prostate cancer epithelial cells that is unrelated to their Ca 2+ binding. BAPTA was the most effective (maximal blockade 67%, IC 50 =70 μM, at +100 mV) followed by EDTA and HEDTA. I Cl,swell blockade by EDTA was pH‐dependent. BAPTA blocked I Cl,swell also in other cell types. We conclude that Ca 2+ chelating agents block I Cl,swell by acting directly on the underlying channel, and that the negative charge of the free chelator form is critical for the blockade.