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Novel splice variants of amphiphysin I are expressed in retina 1
Author(s) -
Terada Yoshiko,
Tsutsui Ken,
Sano Kuniaki,
Hosoya Osamu,
Ohtsuki Hiroshi,
Tokunaga Akira,
Tsutsui Kimiko
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02763-1
Subject(s) - insert (composites) , splice , complementary dna , microbiology and biotechnology , biology , cloning (programming) , exon , gene , alternative splicing , genetics , mechanical engineering , computer science , engineering , programming language
Using RT‐PCR‐based cDNA cloning, we identified novel splice variants of amphiphysin I, termed amph Ir, that are expressed specifically in retina. In comparison with the prototype amphiphysin I, amph Ir contained two novel insertions (inserts A and B) and one deletion. Insert A is only 9 bp in length but appears to be a determinant for the retina‐specific expression. In contrast, insert B is a large domain of 1740 bp and two shorter transcripts with 3′‐truncated insert B were also expressed. All the insert sequences were present as unidentified exons in the amphiphysin I gene on human chromosome 7. Western blot analysis of various rat tissues with anti‐insert B antibody confirmed the presence and tissue specificity of the variant proteins.