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Insulators prevent transcriptional interference between two promoters in a double gene construct for transgenesis
Author(s) -
Hasegawa Kouichi,
Nakatsuji Norio
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02761-8
Subject(s) - transgenesis , transgene , promoter , biology , gene , transfection , gene expression , construct (python library) , transcriptional regulation , computational biology , genetics , microbiology and biotechnology , computer science , reproductive biology , embryogenesis , programming language
In transgenesis, the expression of two transgenes is often subject to mutual interference by each of the two expression cassettes when they are driven by different transcriptional regulatory elements in a single construct. To study this problem, we constructed vectors consisting of two expression units, one contains a strong ubiquitous promoter and the other contains a tissue‐specific transcriptional element. The expression pattern of each transgene was examined in transfected cell lines and also in transgenic mice. In both cases, two expression units in a single construct were expressed in an independent manner and were controlled by their respective regulatory element only if we placed insulators at both ends of one expression unit. These results indicate that usage of insulators is a valuable tool for transfection of double gene constructs in transgenesis.