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A novel striated tropomyosin incorporated into organized myofibrils of cardiomyocytes in cell and organ culture
Author(s) -
Zajdel Robert W.,
Sanger Jean M.,
Denz Christopher R.,
Lee Sung,
Dube Syamalima,
Poiesz Bernard J.,
Sanger Joseph W.,
Dube Dipak K.
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02756-4
Subject(s) - tropomyosin , myofibril , organ culture , myocyte , microbiology and biotechnology , chemistry , actin , biology , biochemistry , in vitro
Striated muscle tropomyosin is classically described as consisting of 10 exons, 1a, 2b, 3, 4, 5, 6b, 7, 8, and 9a/b, in both skeletal and cardiac muscle. A novel isoform found in embryonic axolotl heart maintains exon 9a/b of striated muscle but also has a smooth muscle exon 2a instead of exon 2b. Translation and subsequent incorporation into organized myofibrils, with both isoforms, was demonstrated with green fluorescent protein fusion protein construct. Mutant axolotl hearts lack sufficient tropomyosin in the ventricle and this smooth/straited chimeric tropomyosin was sufficient to replace the missing tropomyosin and form organized myofibrils.