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Identification of o ‐quinone/quinone methide metabolites of quercetin in a cellular in vitro system
Author(s) -
Awad Hanem M.,
Boersma Marelle G.,
Boeren Sjef,
van der Woude Hester,
van Zanden Jelmer,
van Bladeren Peter J.,
Vervoort Jacques,
Rietjens Ivonne M.C.M.
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02754-0
Subject(s) - quinone methide , quercetin , quinone , in vitro , tyrosinase , biochemistry , intracellular , chemistry , metabolism , cell culture , peroxidase , carcinogen , antioxidant , enzyme , biology , genetics
Formation of quercetin quinone/quinone methide metabolites, reflected by formation of the glutathionyl quercetin adducts as authentic metabolites, was investigated in an in vitro cell model (B16F‐10 melanoma cells). Results of the present study clearly indicate the formation of glutathionyl quercetin adducts in a tyrosinase‐containing melanoma cell line, expected to be representative also for peroxidase‐containing mammalian cells and tissues. The data obtained also support that the adducts are formed intracellular and subsequently excreted into the incubation medium and reveal for the first time evidence for the pro‐oxidative metabolism of quercetin in a cellular in vitro model.