Premium
Human septin–septin interaction: CDCrel‐1 partners with KIAA0202
Author(s) -
Bläser Susanne,
Jersch Katrin,
Hainmann Ina,
Wunderle Daniela,
Zgaga-Griesz Andrea,
Busse Anja,
Zieger Barbara
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02749-7
Subject(s) - septin , cytokinesis , immunoprecipitation , gtpase , microbiology and biotechnology , biology , cell division , cytoskeleton , fusion protein , genetics , cell culture , cell , gene , recombinant dna
Septins are evolutionary conserved cytoskeletal GTPases forming heteropolymer complexes involved in cytokinesis and other cellular processes. CDCrel‐1 (cell division cycle related‐1) is a recently cloned and characterized human septin which is highly expressed in non‐dividing cells, such as neurons. Using a yeast two‐hybrid system we demonstrate that CDCrel‐1 partners with another uncharacterized human septin, KIAA0202. The interaction of CDCrel‐1 and KIAA0202 was confirmed in the human leukemia cell line K‐562 using pull‐down assays with a KIAA0202–glutathione S ‐transferase fusion protein and by immunoprecipitation of the CDCrel‐1–KIAA0202 complex with an anti‐KIAA0202 antibody. Expression studies of the two human septins revealed a concomitant expression of both proteins in certain cells.