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Identification of a novel prokaryotic HEAT‐repeats‐containing protein which interacts with a cyanobacterial IscA homolog
Author(s) -
Morimoto Kozo,
Nishio Kazuaki,
Nakai Masato
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02736-9
Subject(s) - chemistry , cyanobacteria , cluster (spacecraft) , biochemistry , homologous chromosome , biology , genetics , computational biology , gene , bacteria , computer science , programming language
IscA homologs are known to be involved in iron–sulfur cluster formation in various organisms. Recombinant proteins of two IscA homologs from the cyanobacterium Synechocystis PCC 6803, designated SLR1417 and SLR1565, were purified. The absorption spectrum of purified SLR1565 was typical for [2Fe–2S] cluster‐containing proteins, whereas that of SLR1417 predominantly showed the presence of the iron ion alone. In the cyanobacterial cell extracts, only SLR1565 was found to form a complex with a novel prokaryotic HEAT‐repeats‐containing protein, SLR1098. Thus, the two cyanobacterial IscA protein homologs exist in distinct molecular states, suggesting different cellular roles for these proteins.