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Partially structured state of the functional VH domain of the mouse anti‐ferritin antibody F11
Author(s) -
Martsev Sergey P,
Dubnovitsky Anatoly P,
Stremovsky Oleg A,
Chumanevich Alexander A,
Tsybovsky Yaroslav I,
Kravchuk Zinaida I,
Deyev Sergey M
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02696-0
Subject(s) - immunoglobulin light chain , antibody , ferritin , chemistry , monoclonal antibody , domain (mathematical analysis) , heavy chain , microbiology and biotechnology , biophysics , computational biology , biochemistry , biology , genetics , mathematical analysis , mathematics
An antibody combining site generally involves the two variable domains, VH from the heavy and VL from the light chain. We expressed the individual VH domain of the mouse anti‐human ferritin monoclonal antibody F11. The loss of affinity was not dramatic ( K a =4.0×10 7 M −1 versus 8.6×10 8 M −1 for the parent antibody) and comparable to that previously observed for other VHs. However, the functional VH domain adopted a partially structured state with a significant amount of distorted secondary and compact yet greatly destabilized tertiary structures, as demonstrated by spectroscopic and calorimetric probes. These data provide the first description for a functional antibody domain that meets all the criteria of a partially structured state.