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PspE (phage‐shock protein E) of Escherichia coli is a rhodanese
Author(s) -
Adams Hendrik,
Teertstra Wieke,
Koster Margot,
Tommassen Jan
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02695-9
Subject(s) - rhodanese , escherichia coli , sulfurtransferase , operon , thiosulfate , bacteria , chemistry , biology , biochemistry , microbiology and biotechnology , gene , genetics , enzyme , sulfur , organic chemistry , cysteine
The psp ( hage‐ hock rotein) operon of Escherichia coli is induced when the bacteria are infected by filamentous phage and under several other stress conditions. The physiological role of the individual Psp proteins is still not known. We demonstrate here that the last gene of the operon, pspE , encodes a thiosulfate:cyanide sulfurtransferase (EC 2.8.1.1; rhodanese). Kinetic analysis revealed that catalysis occurs via a double displacement mechanism as described for other rhodaneses. The K m s for SSO 3 2− and CN − were 4.6 and 27 mM, respectively.