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Catalase negative Staphylococcus aureus retain virulence in mouse model of chronic granulomatous disease
Author(s) -
Messina Carlo G.M,
Reeves Emer P,
Roes Jürgen,
Segal Anthony W
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02658-3
Subject(s) - chronic granulomatous disease , catalase , staphylococcus aureus , virulence , microbiology and biotechnology , nadph oxidase , oxidase test , myeloperoxidase , bacteria , biology , virulence factor , enzyme , chemistry , immunology , biochemistry , gene , genetics , inflammation
Myeloperoxidase‐mediated chlorination is thought to be a necessary microbicidal mechanism. The H 2 O 2 required for this process is generated by the NADPH oxidase. Staphylococcus aureus can also produce H 2 O 2 , which is not broken down by catalase negative organisms. It has been thought that this bacterial H 2 O 2 can substitute for cellular H 2 O 2 in the halogenation reaction in chronic granulomatous disease (CGD) where neutrophils are lacking the NADPH oxidase. We have readdressed this issue in a mouse model of CGD using clinical isolates of catalase positive and negative strains of S. aureus . The results showed these organisms to be equally virulent and that the H 2 O 2 they produced is insufficient to cause significant iodination, a marker for chlorination, thereby contradicting the accepted views on this subject.