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A nuclear transcription factor related to plastid ribosome biogenesis is synthesised early during germination and priming
Author(s) -
Achard Patrick,
Job Dominique,
Mache Régis
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02642-x
Subject(s) - imbibition , plastid , germination , ribosome biogenesis , biology , gene , priming (agriculture) , transcription factor , ribosomal protein , microbiology and biotechnology , transcription (linguistics) , gene expression , ribosome , promoter , biogenesis , genetics , botany , rna , chloroplast , linguistics , philosophy
Germination is a short developmental process during which many new proteins are synthesised. We have chosen the previously characterised RPL21 gene encoding plastid‐localised ribosomal proteins RPL21, to analyse activation of gene expression during germination. Transcription activation occurs at the P1 promoter during the first hours following imbibition and coincides with the appearance of a trans ‐acting factor that we named AUBE1. AUBE1 binds specifically to a short DNA fragment that encompasses the P1 promoter of the RPL21 gene. The protein has a size of 28–30 kDa and is transiently expressed during the early phase of germination. Using the properties of primed seeds we show that AUBE1 is maintained after desiccation of primed seeds. We conclude that AUBE1 can be used as a marker in spinach seed priming.