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The biosynthetic pathway for lipoic acid is present in plastids and mitochondria in Arabidopsis thaliana 1
Author(s) -
Yasuno Rie,
Wada Hajime
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02589-9
Subject(s) - plastid , lipoic acid , arabidopsis thaliana , chloroplast , mitochondrion , biochemistry , biology , biosynthesis , atp synthase , mutant , arabidopsis , retrograde signaling , enzyme , gene , antioxidant
In eukaryotes, the biosynthetic pathway for lipoic acid is present in mitochondria. However, it has been hypothesized that, in plants, the biosynthetic pathway is present in plastids in addition to mitochondria. In this study, Arabidopsis thaliana LIP1p cDNA for a plastidial form of lipoic acid synthase has been identified. We show that it encodes a lipoic acid synthase by demonstrating its ability to complement an Escherichia coli mutant lacking lipoic acid synthase activity. We also show that LIP1p is targeted to chloroplasts. These findings suggest that the biosynthetic pathway for lipoic acid is present not only in mitochondria but also in plastids.