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Aerobic and anaerobic NAD + metabolism in Saccharomyces cerevisiae
Author(s) -
Panozzo Cristina,
Nawara Magdalena,
Suski Catherine,
Kucharczyk Roza,
Skoneczny Marek,
Bécam Anne-Marie,
Rytka Joanna,
Herbert Christopher J
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02585-1
Subject(s) - saccharomyces cerevisiae , quinolinate , biochemistry , auxotrophy , kynurenine , nad+ kinase , kynurenine pathway , chemistry , tryptophan , enzyme , anthranilic acid , transferase , metabolic pathway , biology , gene , amino acid , quinolinic acid , mutant
In Saccharomyces cerevisiae the nicotinic acid moiety of NAD + can be synthesized from tryptophan using the kynurenine pathway or incorporated directly using nicotinate phosphoribosyl transferase ( NPT1 ). We have identified the genes that encode the enzymes of the kynurenine pathway and for BNA5 (YLR231c) and BNA6 (YFR047c) confirmed that they encode kynureninase and quinolinate phosphoribosyl transferase respectively. We show that deletion of genes encoding kynurenine pathway enzymes are co‐lethal with the Δ npt1 , demonstrating that no other pathway for the synthesis of nicotinic acid exists in S. cerevisiae . Also, we show that under anaerobic conditions S. cerevisiae is a nicotinic acid auxotroph.