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Cold stress decreases the capacity for respiratory NADH oxidation in potato leaves
Author(s) -
Svensson Å.Staffan,
Johansson Fredrik I,
Møller Ian M,
Rasmusson Allan G
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02581-4
Subject(s) - alternative oxidase , respiratory chain , nadh dehydrogenase , gene , biochemistry , rotenone , solanum tuberosum , dehydrogenase , biology , enzyme , mitochondrion , yeast , escherichia coli , chemistry , mitochondrial dna , botany
Cold stress effects on the expression of genes for respiratory chain enzymes were investigated in potato ( Solanum tuberosum L., cv. Desiree) leaves. The nda 1 and ndb 1 genes, homologues to genes encoding the non‐proton‐pumping respiratory chain NADH dehydrogenases of Escherichia coli and yeast, were compared to genes encoding catalytic subunits of the proton‐pumping NADH dehydrogenase (complex I). Using a real‐time PCR system, we demonstrate a specific and gradual decrease of the NDA1 transcript after exposing the plants to 5°C. After 6 days of cold treatment the NDA1 transcript abundance is 10% of the original level. This decrease is accompanied by specific decreases of immunodetected NDA protein and internal rotenone‐insensitive NADH oxidation in mitochondria isolated from cold‐treated plants. The alternative oxidase is not cold‐induced neither at the protein nor at the activity level. The results are discussed in relation to the recent finding that the nda 1 gene expression is completely light‐dependent.