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Involvement of fibrinogen specific binding in erythrocyte aggregation
Author(s) -
Lominadze David,
Dean William L
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02575-9
Subject(s) - fibrinogen , erythrocyte aggregation , erythrocyte membrane , chemistry , biochemistry , binding site , plasma protein binding , red blood cell , biophysics , membrane , biology
Increased fibrinogen concentration and erythrocyte aggregation are significant risk factors during various cardiovascular diseases and cerebrovascular disorders. Currently, fibrinogen‐induced erythrocyte aggregation is thought to be caused by a non‐specific binding mechanism. However, the published data on changes in erythrocyte aggregation during hypertension point to the possible existence of other mechanism(s). Therefore, we tested the hypothesis that specific binding of fibrinogen is involved in erythrocyte aggregation. It was found that Oregon Green 488‐labeled human fibrinogen specifically binds rat erythrocyte membranes with a K d of 1.3 μM. Further experiments showed that the peptide Arg‐Gly‐Asp‐Ser blocked both fibrinogen‐induced aggregation of intact erythrocytes and specific binding of fibrinogen to the erythrocyte membranes. These results suggest that in addition to non‐specific binding, a specific binding mechanism is also involved in fibrinogen‐induced erythrocyte aggregation.