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CLK‐1 protein has DNA binding activity specific to O L region of mitochondrial DNA
Author(s) -
Gorbunova Vera,
Seluanov Andrei
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02568-1
Subject(s) - mitochondrial dna , biology , caenorhabditis elegans , gene , genetics , dna , dna replication , transcription (linguistics) , dna binding protein , microbiology and biotechnology , transcription factor , linguistics , philosophy
Mutations in the clk‐1 gene of Caenorhabditis elegans extend worm life span and slow down a variety of physiological processes. Here we report that C. elegans CLK‐1 as well as its mouse homologue have DNA binding activity that is specific to the O L region of mitochondrial DNA. DNA binding activity of CLK‐1 is inhibited by ADP, and is altered by mutations that extend nematode life span. Our results suggest that, in addition to its enzymatic function in ubiquinone biosynthesis, CLK‐1 is involved in the regulation of mtDNA replication or transcription.