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Der f 16: a novel gelsolin‐related molecule identified as an allergen from the house dust mite, Dermatophagoides farinae 1
Author(s) -
Kawamoto Seiji,
Suzuki Takayuki,
Aki Tsunehiro,
Katsutani Takashi,
Tsuboi Shinji,
Shigeta Seiko,
Ono Kazuhisa
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02540-1
Subject(s) - gelsolin , house dust mite , allergen , recombinant dna , immunoglobulin e , mite , immunology , allergy , chemistry , antigen , biology , microbiology and biotechnology , antibody , biochemistry , actin , gene , botany
Allergen from the house dust mite ( Dermatophagoides sp.) is a major trigger factor of allergic disorders, and its characterization is crucial for the development of specific diagnosis or immunotherapy. Here we report the identification of a novel dust mite ( Dermatophagoides farinae ) antigen whose primary structure belongs to the gelsolin family, a group of actin cytoskeleton‐regulatory proteins. Isolated mite cDNA, termed Der f 16, encodes 480 amino acids comprising a four‐repeated gelsolin‐like segmental structure, which is not seen in conventional gelsolin family members. Enzyme immunoassay indicated that recombinant Der f 16 protein, prepared using an Escherichia coli expression system, bound IgE from mite‐allergic patients at 47% (8/17) frequency. This is the first evidence that the gelsolin family represents a new class of allergen recognizable by atopic patient IgE.