Premium
The plant Rad50–Mre11 protein complex
Author(s) -
Daoudal-Cotterell S,
Gallego M.E,
White C.I
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02536-x
Subject(s) - rad50 , immunoprecipitation , arabidopsis thaliana , biology , arabidopsis , dna repair , telomere binding protein , microbiology and biotechnology , dna replication , homologous recombination , saccharomyces cerevisiae , telomere , dna , genetics , dna binding protein , yeast , gene , mutant , transcription factor
The Rad50–Mre11–Xrs2/Nbs1 protein complex plays critical roles in cellular processes involving DNA ends. This complex is implicated in DNA recombination and replication, meiosis, telomere maintenance and cellular DNA damage responses. The Rad50 and Mre11 proteins are essential for viability in animals, although not in yeast. We have prepared antibodies to the Rad50 protein of the model plant Arabidopsis thaliana which recognize a 175 kDa protein in wild‐type Arabidopsis protein extracts. Furthermore, we report here demonstration of the existence of the Rad50–Mre11 complex by co‐immunoprecipitation of the Rad50 and Mre11 proteins from the plant cell extracts.