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Identification of ten exon deleted ERβ mRNAs in human ovary, breast, uterus and bone tissues: alternate splicing pattern of estrogen receptor β mRNA is distinct from that of estrogen receptor α
Author(s) -
Poola Indra,
Abraham Jessy,
Baldwin Kate
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02521-8
Subject(s) - exon , tandem exon duplication , exon trapping , alternative splicing , biology , rna splicing , microbiology and biotechnology , exon shuffling , splice site mutation , estrogen receptor , estrogen receptor alpha , genetics , gene , rna , breast cancer , cancer
Four different human tissues and breast cancer cell lines were screened to identify exon deletion variant transcripts of estrogen receptor β (ERβ) by reverse transcription‐polymerase chain reaction using the ‘splice targeted primer approach’ that amplifies each category of exon deleted variants as a separate gene population. A total of 10 different variant mRNAs that have deletions in various combination of exons were identified by sequence analysis. They were exon 2Δ; exons 2 and 5–6Δ; exon 3Δ; exon 4Δ; exon 5Δ; exons 5 and 2Δ; exon 6Δ; exons 6 and 2Δ; exons 6, 2–3Δ; and exons 5–6Δ. In some cases, deletion of an exon appears to be associated with a mutation of a specific base. Although ERα and ERβ are highly homologous, have identical exon and functional domain organization, exhibit similar ligand‐binding profiles and interact with identical DNA response elements, the sequence of exon skipping in ERβ pre‐mRNA appears to be distinct from that of ERα mRNA. Furthermore, results described here also suggest that alternate splicing of ERβ mRNA is tissue specific. The presence of a ERβ variant profile together with other ER isoforms in a tissue may have functional implications in binding and response to a particular ligand.