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β‐Arrestin is involved in the desensitization but not in the internalization of the somatostatin receptor 2A expressed in CHO cells
Author(s) -
Brasselet Sabrina,
Guillen Stéphanie,
Vincent Jean-Pierre,
Mazella Jean
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02517-6
Subject(s) - internalization , somatostatin , transfection , arrestin , receptor , somatostatin receptor , microbiology and biotechnology , chinese hamster ovary cell , desensitization (medicine) , somatostatin receptor 2 , endosome , chemistry , vesicle , g protein coupled receptor , biology , neuroscience , biochemistry , gene , membrane
The interaction of β‐arrestin‐1 with the somatostatin receptor type 2A (sst2A) was monitored using both biochemical and confocal imaging approaches. We show that, using transient transfection of either β‐arrestin‐1 or its dominant negative Δ‐arrestin‐1 in CHO cells stably transfected with the sst2A, β‐arrestin‐1 is colocalized with the receptor in endosomal vesicles after somatostatin‐induced sequestration. However, this interaction leads to a role of β‐arrestin‐1 in the desensitization of the sst2A rather than in the internalization process of the receptor–ligand complex.