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The Fluorosome™ technique for investigating membrane on‐ and off‐loading of drugs by β‐CD and sonicated SUV
Author(s) -
Fix Marina,
Melchior Donald L
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02512-7
Subject(s) - phloretin , chemistry , bilayer , membrane , fluorophore , fluorescence , liposome , lipid bilayer , sonication , phosphatidylcholine , chromatography , phospholipid , vesicle , quenching (fluorescence) , biophysics , biochemistry , biology , physics , quantum mechanics
The application of the Fluorosome technique to test drug delivery systems is described. Fluorosomes, egg phosphatidylcholine liposomes with bilayer embedded fluorophores, were employed to investigate the ability of sonicated small unilamellar vesicles (sSUV) and β‐cyclodextrins (β‐CD) to deliver drugs into or extract drugs from the fluorosome's phospholipid bilayer. The addition of phloretin to a fluorosome suspension resulted in fluorescence reduction reflecting phloretin entering the bilayer and quenching fluorophore fluorescence. Subsequent addition of sSUV to phloretin pretreated fluorosomes showed an increase in fluorescence reflecting phloretin extraction from the fluorosome membrane. Sequential additions of β‐estradiol loaded β‐CD to fluorosomes as well as the addition of β‐estradiol alone resulted in fluorescence reduction due to β‐estradiol insertion into the membrane. Further addition of pure β‐CD resulted in a fluorescence increase indicating β‐estradiol extraction from the fluorosome membrane.