Premium
Presence of a novel subset of NKT cells bearing an invariant Vα19.1–Jα26 TCR α chain
Author(s) -
Shimamura Michio,
Huang Yi-Ying
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02509-7
Subject(s) - cd1d , natural killer t cell , t cell receptor , cd1 , microbiology and biotechnology , biology , antigen , t cell , immunology , chemistry , immune system
CD1d‐deficient (CD1d −/− ) mouse lymphocytes were analyzed to classify the natural killer T (NKT) cells without reactivity to CD1d. The cells bearing a Vα19.1–Jα26 (AV19–AJ33) invariant TCR α chain, originally found in the peripheral blood lymphocytes, were demonstrated to be abundant in the NK1.1 + but not NK1.1 − T cell population isolated from CD1d −/− mice. Moreover, more than half (11/21) of the hybrid cell lines established from CD1d −/− NKT cells expressed the Vα19.1–Jα26 invariant TCR α chain. The expression of the invariant Vα19.1–Jα26 mRNA was absent in β2‐microglobulin‐deficient mice. Collectively, the present findings suggest the presence of a second NKT cell repertoire characterized by an invariant TCR α chain (Vα19.1–Jα26) that is selected by an MHC class I‐like molecule other than CD1d.