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Differentiation of single‐cell organisms according to elongation stages crucial for gene expression efficacy
Author(s) -
Likhoshvai Vitali A.,
Matushkin Yuri G.
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02507-3
Subject(s) - elongation , gene expression , microbiology and biotechnology , gene , cell , biology , cellular differentiation , computational biology , chemistry , genetics , materials science , ultimate tensile strength , metallurgy
We analyzed the interrelation between the efficiency of a gene expression and the nucleotide composition of all protein‐coding sequences in 38 unicellular organisms whose complete genomic sequences are known. These organisms comprise 37 prokaryotic (29 eubacteria and eight archaebacteria) and one eukaryotic (yeast) species. We demonstrated that frequency analysis of gene codon composition fails to reflect adequately the gene expression efficiency of all these organisms. We constructed a measure, the elongation efficiency index, that considers simultaneously the information on codon frequencies and the degree of mRNA local self‐complementarity. This measure recognizes the ribosome‐coding genes as highly expressed in all the unicellular organisms studied. According to our analysis, these species fall into five groups differentiated by the process that makes the key contribution to the elongation rate.