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Targeted cytosolic delivery of hydrogel nanoparticles into HepG2 cells through engineered Sendai viral envelopes
Author(s) -
Jana Siddhartha S,
Bharali Dhruba J,
Mani Prashant,
Maitra Amarnath,
Gupta Chhitar M,
Sarkar Debi P
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02467-5
Subject(s) - sendai virus , chemistry , cytosol , biophysics , fluorescence microscope , in vitro , cytoplasm , in vivo , microbiology and biotechnology , biochemistry , fluorescence , biology , enzyme , physics , quantum mechanics , gene
Hydrogel nanoparticles of cross‐linked polyvinylpyrrolidone (PVP‐NP) (35–50 nm in diameter) containing fluoresceinated dextran (FITC‐Dx) were encapsulated in reconstituted Sendai viral envelopes containing only the fusion (F) protein (F‐virosomes 1 ). Incubation of these loaded F‐virosomes with human hepatoblastoma cells (HepG2) in culture resulted in membrane‐fusion‐mediated delivery of NPs to the cell cytoplasm, as inferred from the ability of cells to internalize FITC‐Dx loaded PVP‐NP (PVP f ‐NP) in the presence of azide (an inhibitor of the endocytotic process). Introduction of PVP f ‐NP into the HepG2 cells was assured by selective accumulation of FITC fluorescence in the cytosolic compartment. The structural integrity of the internalized PVP f ‐NP was also confirmed by fluorescence microscopy and ultracentrifugation analysis. The potential usefulness of PVP‐NP‐mediated cytosolic release of water soluble drugs both in vitro and in vivo has been established for the first time.