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Nitric oxide affects plant mitochondrial functionality in vivo
Author(s) -
Zottini Michela,
Formentin Elide,
Scattolin Michela,
Carimi Francesco,
Lo Schiavo Fiorella,
Terzi Mario
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02438-9
Subject(s) - mitochondrion , cytochrome c oxidase , nitric oxide , mitochondrial apoptosis induced channel , alternative oxidase , cytochrome c , microbiology and biotechnology , respiration , apoptosome , programmed cell death , membrane potential , cytochrome , chemistry , cell , inner mitochondrial membrane , biochemistry , biophysics , apoptosis , biology , enzyme , botany , caspase , organic chemistry
In this report, we show that nitric oxide affects mitochondrial functionality in plant cells and reduces total cell respiration due to strong inhibition of the cytochrome pathway. The residual respiration depends on the alternative pathway and novel synthesis of alternative oxidase occurs. These modifications are associated with depolarisation of the mitochondrial membrane potential and release of cytochrome c from mitochondria, suggesting a conserved signalling pathway in plants and animals. This signal cascade is triggered at the mitochondrial level and induces about 20% of cell death. In order to achieve a higher level of cell death, the addition of H 2 O 2 is necessary.