Premium
Involvement of phosphatidylinositol 3‐kinase in nuclear translocation of protein kinase C ζ induced by C2‐ceramide in rat hepatocytes
Author(s) -
Calcerrada M.C,
Miguel B.G,
Martı́n L,
Catalán R.E,
Martı́nez A.M
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02401-8
Subject(s) - ceramide , wortmannin , protein kinase c , phosphatidylinositol , cytosol , kinase , microbiology and biotechnology , lipid signaling , chromosomal translocation , biology , chemistry , biochemistry , apoptosis , enzyme , gene
In this study we report that protein kinase C ζ (PKC ζ), one of the atypical isoforms of the PKC family located predominantly in cytosol, is redistributed by C2‐ceramide treatment in isolated hepatocytes. PKC ζ increased in membrane and nuclear fractions after 30 min of treatment with C2‐ceramide in a dose‐ and time‐dependent manner. The action of C2‐ceramide was inhibited by wortmannin and LY 294002, indicating that C2‐ceramide‐induced PKC ζ increase in both nucleus and membrane fractions is mediated by phosphatidylinositol 3‐kinase (PI3‐kinase) activation. In addition, a significant translocation of PI3‐kinase to the nucleus was observed after C2‐ceramide treatment.