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Regulation of fibronectin alternative splicing by a basement membrane‐like extracellular matrix
Author(s) -
Srebrow Anabella,
Blaustein Matı́as,
Kornblihtt Alberto R
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02382-7
Subject(s) - fibronectin , laminin , extracellular matrix , basement membrane , microbiology and biotechnology , alternative splicing , tenascin , extracellular , chemistry , matrix (chemical analysis) , exon , biology , gene , biochemistry , chromatography
Hepatocytes are the source of plasma fibronectin (FN) which lacks the alternatively spliced EDI segment, distinctive of oncofetal FN. When hepatic or other epithelial cells are cultured on plastic, EDI inclusion is triggered. Here we report that EDI inclusion is inhibited when hepatic cells are cultured on a basement membrane‐like extracellular matrix (ECM), demonstrating a new role for the ECM in the control of gene expression. The effect is duplicated by collagen IV and laminin but not by collagen I; is not observed with another alternatively spliced FN exon (EDII); and correlates with a decrease in cell proliferation, consistently with high EDI inclusion levels observed in many physiological and pathological proliferative processes.