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The advantages of cDNA microarray as an effective tool for identification of reproductive organ‐specific genes in a model legume, Lotus japonicus
Author(s) -
Endo Makoto,
Matsubara Hitoshi,
Kokubun Takao,
Masuko Hiromi,
Takahata Yoshihito,
Tsuchiya Tohru,
Fukuda Hiroo,
Demura Taku,
Watanabe Masao
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02371-2
Subject(s) - complementary dna , biology , gynoecium , microarray , cdna library , microarray analysis techniques , gene , gene chip analysis , identification (biology) , lotus japonicus , lotus , stamen , genetics , computational biology , gene expression , botany , pollen , mutant
To understand the molecular mechanisms intrinsic to reproductive organ development a cDNA microarray, fabricated from flower bud cDNA clones, was used to isolate genes, which are specifically expressed during the development of the anther and pistil in Lotus japonicus . Cluster analysis of the microarray data revealed 21 and 111 independent cDNA groups, which were specifically expressed in immature and mature anthers, respectively. RT‐PCR was performed to provide a direct assessment of the accuracy and reproducibility of our approach. Confirmation of our results suggests that cDNA microarray technology is an effective tool for identification of novel reproductive organ‐specific genes.