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The actin‐binding protein Filamin‐A interacts with the metabotropic glutamate receptor type 7
Author(s) -
Enz Ralf
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02361-x
Subject(s) - filamin , metabotropic glutamate receptor 1 , metabotropic glutamate receptor , metabotropic glutamate receptor 7 , metabotropic glutamate receptor 5 , microbiology and biotechnology , chemistry , biochemistry , biology , glutamate receptor , receptor , cytoskeleton , cell
A yeast two‐hybrid screen identified Filamin‐A as a binding partner of the metabotropic glutamate receptor type 7b (mGluR7b) splice variant. In addition, Filamin‐A interacted with mGluR4a, mGluR5a, mGluR5b, mGluR7a and mGluR8a. Domain mapping revealed that alternative splicing of mGluR4, mGluR7 and mGluR8 C‐termini regulated the interaction. A conserved tyrosine within mGluR C‐termini was identified to mediate the binding to Filamin‐A. Protein interactions were verified in biochemical assays using recombinant and native proteins. Finally, co‐expression of Filamin‐A and mGluR7 splice variants was shown in brain regions. These findings suggest that Filamin‐A may physically link metabotropic glutamate receptors to the actin cytoskeleton.